Tourism in protected areas is increasingly important to the economy of environmentally vulnerable countries. However, when pursuing sustainable tourism in protected areas, balancing the benefits of tourism with its associated impacts, including the increasing access (mainly through roads) to the last wilderness refuges, remains a challenge. Current projections of ecotourism development suggest a significant increase in the road network–both paved and unpaved roads–, as well as other improvements to the infrastructure network, within and between protected areas. In protected areas with limited freedom for people to walk or be outside vehicles, as in those African parks where the main attraction are self-driven safaris, river crossings are some of the main places where people can interact with and come close to wildlife. One of the greatest negative consequences of the massive increase in tourism in protected areas is the habituation of wildlife to humans, implying the loss of their perception of these tourists as potential predators. If confirmed, feeding by tourists could dramatically increase the risk of wildlife road-kill, one of the most important negative impacts of the car-dependent tourism.