Road maintenance provides us excellent opportunities to reduce road’s impacts on wildlife as well as road-wildlife hazards. Mitigation measures maintenance also play a key role for ensuring their long-term effectiveness. Current practices in Europe were analyzed conducting interviews to road managers and performing a literature review within the framework of SAFEROAD project, financed by Conference of European Director of Roads. Based on this information Guidelines for Best Maintenance Practices are being drafted to provide guidance on road maintenance policies, procedures and practices with regard to wildlife conservation while guaranteeing maintenance and road safety standards.