Roads are a main cause of habitat fragmentation but mitigating the full road network is unfeasible. A key goal in the road mitigation planning process is to highlight, at the transportation network level, the most problematic roads, i.e. where mitigation measures are most required in order to maximize the benefits for biodiversity while keeping implementation costs as low as possible. Grounded on the concepts of habitat amount and accessible habitat, we prioritized roads for mitigation based on dual spatial graphs, where the land polygons delimited by roads are the nodes and the roads themselves are the links. The rationale was to identify those links (roads) that connect the nodes with higher potential biodiversity (as a proxy for quality habitat). We applied this approach to prioritize the defragmentation of the major road network of the Iberian Peninsula, targeting all native mammalian carnivores inhabiting this region …