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en Metabolomic responses in caged clams, Ruditapes decussatus, exposed to agricultural and urban inputs in a Mediterranean coastal lagoon (Mar Menor, SE Spain) -
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del recurso Distribución HTML a2026-06-25
(anteriormente2026-06-23
) en Metabolomic responses in caged clams, Ruditapes decussatus, exposed to agricultural and urban inputs in a Mediterranean coastal lagoon (Mar Menor, SE Spain)
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| 95 | "notes": "The Mar Menor is a coastal lagoon affected by the growth | 105 | "notes": "The Mar Menor is a coastal lagoon affected by the growth | ||
| 96 | of intensive agriculture and urban development in the surrounding | 106 | of intensive agriculture and urban development in the surrounding | ||
| 97 | area. Large amounts of chemical pollutants from these areas are | 107 | area. Large amounts of chemical pollutants from these areas are | ||
| 98 | discharged into El Albujon, a permanent water-course flowing into the | 108 | discharged into El Albujon, a permanent water-course flowing into the | ||
| 99 | lagoon. Biomarkers such as the activity of acetylcholinesterase or | 109 | lagoon. Biomarkers such as the activity of acetylcholinesterase or | ||
| 100 | antioxidant enzymes have been previously tested in this lagoon | 110 | antioxidant enzymes have been previously tested in this lagoon | ||
| 101 | demonstrating the presence of neurotoxicity and oxidative stress in | 111 | demonstrating the presence of neurotoxicity and oxidative stress in | ||
| 102 | clams transplanted in sites affected by the dispersion of the effluent | 112 | clams transplanted in sites affected by the dispersion of the effluent | ||
| 103 | from El Albujon. To complete this traditional toxicology work, a | 113 | from El Albujon. To complete this traditional toxicology work, a | ||
| 104 | metabolomic profiling of these transplanted organisms has been carried | 114 | metabolomic profiling of these transplanted organisms has been carried | ||
| 105 | out for the detection of metabolic biomarkers induced by | 115 | out for the detection of metabolic biomarkers induced by | ||
| 106 | agricultural/urban pollutants. More than 70 metabolites have been | 116 | agricultural/urban pollutants. More than 70 metabolites have been | ||
| 107 | quantified using a targeting metabolomics platform based on HPLC-MS. | 117 | quantified using a targeting metabolomics platform based on HPLC-MS. | ||
| 108 | The intracellular metabolic pattern was analyzed by PCA from the | 118 | The intracellular metabolic pattern was analyzed by PCA from the | ||
| 109 | digestive gland of clams after 7 and 22 days of transplantation. | 119 | digestive gland of clams after 7 and 22 days of transplantation. | ||
| 110 | Results showed a different profile of metabolite between organisms | 120 | Results showed a different profile of metabolite between organisms | ||
| 111 | collected from control and exposed sites. At the shorter exposure | 121 | collected from control and exposed sites. At the shorter exposure | ||
| 112 | time, there was an increase in several metabolites in the latter when | 122 | time, there was an increase in several metabolites in the latter when | ||
| 113 | compared with those from control sites, whereas metabolic profiling at | 123 | compared with those from control sites, whereas metabolic profiling at | ||
| 114 | 22 days showed that those metabolites were drastically diminished, | 124 | 22 days showed that those metabolites were drastically diminished, | ||
| 115 | with even lower levels than at control sites. These metabolites | 125 | with even lower levels than at control sites. These metabolites | ||
| 116 | included: (i) 12 amino acids from the 21 proteogenic and HomoSer, (ii) | 126 | included: (i) 12 amino acids from the 21 proteogenic and HomoSer, (ii) | ||
| 117 | osmotic protectants such as gamma-butyrobetaine and taurine and (iii) | 127 | osmotic protectants such as gamma-butyrobetaine and taurine and (iii) | ||
| 118 | nucleotides such as ITP. Regarding sulfur-containing molecules, | 128 | nucleotides such as ITP. Regarding sulfur-containing molecules, | ||
| 119 | taurine could be highlighted as a potential biomarker since its | 129 | taurine could be highlighted as a potential biomarker since its | ||
| 120 | concentration was reduced by more than 30 times after 22 days of | 130 | concentration was reduced by more than 30 times after 22 days of | ||
| 121 | exposure, whereas the antioxidant glutathione remained constant in the | 131 | exposure, whereas the antioxidant glutathione remained constant in the | ||
| 122 | organisms from both control and exposed sites. Although targeted | 132 | organisms from both control and exposed sites. Although targeted | ||
| 123 | metabolomics has been shown as an early technique of pollutant effect | 133 | metabolomics has been shown as an early technique of pollutant effect | ||
| 124 | detection, the two-phase pattern could highlight a more complicated | 134 | detection, the two-phase pattern could highlight a more complicated | ||
| 125 | metabolite response to pollutants than classical biomarkers. (C) 2015 | 135 | metabolite response to pollutants than classical biomarkers. (C) 2015 | ||
| 126 | Elsevier B.V. All rights reserved.", | 136 | Elsevier B.V. All rights reserved.", | ||
| 127 | "notes_translated": { | 137 | "notes_translated": { | ||
| 128 | "es": "The Mar Menor is a coastal lagoon affected by the growth of | 138 | "es": "The Mar Menor is a coastal lagoon affected by the growth of | ||
| 129 | intensive agriculture and urban development in the surrounding area. | 139 | intensive agriculture and urban development in the surrounding area. | ||
| 130 | Large amounts of chemical pollutants from these areas are discharged | 140 | Large amounts of chemical pollutants from these areas are discharged | ||
| 131 | into El Albujon, a permanent water-course flowing into the lagoon. | 141 | into El Albujon, a permanent water-course flowing into the lagoon. | ||
| 132 | Biomarkers such as the activity of acetylcholinesterase or antioxidant | 142 | Biomarkers such as the activity of acetylcholinesterase or antioxidant | ||
| 133 | enzymes have been previously tested in this lagoon demonstrating the | 143 | enzymes have been previously tested in this lagoon demonstrating the | ||
| 134 | presence of neurotoxicity and oxidative stress in clams transplanted | 144 | presence of neurotoxicity and oxidative stress in clams transplanted | ||
| 135 | in sites affected by the dispersion of the effluent from El Albujon. | 145 | in sites affected by the dispersion of the effluent from El Albujon. | ||
| 136 | To complete this traditional toxicology work, a metabolomic profiling | 146 | To complete this traditional toxicology work, a metabolomic profiling | ||
| 137 | of these transplanted organisms has been carried out for the detection | 147 | of these transplanted organisms has been carried out for the detection | ||
| 138 | of metabolic biomarkers induced by agricultural/urban pollutants. More | 148 | of metabolic biomarkers induced by agricultural/urban pollutants. More | ||
| 139 | than 70 metabolites have been quantified using a targeting | 149 | than 70 metabolites have been quantified using a targeting | ||
| 140 | metabolomics platform based on HPLC-MS. The intracellular metabolic | 150 | metabolomics platform based on HPLC-MS. The intracellular metabolic | ||
| 141 | pattern was analyzed by PCA from the digestive gland of clams after 7 | 151 | pattern was analyzed by PCA from the digestive gland of clams after 7 | ||
| 142 | and 22 days of transplantation. Results showed a different profile of | 152 | and 22 days of transplantation. Results showed a different profile of | ||
| 143 | metabolite between organisms collected from control and exposed sites. | 153 | metabolite between organisms collected from control and exposed sites. | ||
| 144 | At the shorter exposure time, there was an increase in several | 154 | At the shorter exposure time, there was an increase in several | ||
| 145 | metabolites in the latter when compared with those from control sites, | 155 | metabolites in the latter when compared with those from control sites, | ||
| 146 | whereas metabolic profiling at 22 days showed that those metabolites | 156 | whereas metabolic profiling at 22 days showed that those metabolites | ||
| 147 | were drastically diminished, with even lower levels than at control | 157 | were drastically diminished, with even lower levels than at control | ||
| 148 | sites. These metabolites included: (i) 12 amino acids from the 21 | 158 | sites. These metabolites included: (i) 12 amino acids from the 21 | ||
| 149 | proteogenic and HomoSer, (ii) osmotic protectants such as | 159 | proteogenic and HomoSer, (ii) osmotic protectants such as | ||
| 150 | gamma-butyrobetaine and taurine and (iii) nucleotides such as ITP. | 160 | gamma-butyrobetaine and taurine and (iii) nucleotides such as ITP. | ||
| 151 | Regarding sulfur-containing molecules, taurine could be highlighted as | 161 | Regarding sulfur-containing molecules, taurine could be highlighted as | ||
| 152 | a potential biomarker since its concentration was reduced by more than | 162 | a potential biomarker since its concentration was reduced by more than | ||
| 153 | 30 times after 22 days of exposure, whereas the antioxidant | 163 | 30 times after 22 days of exposure, whereas the antioxidant | ||
| 154 | glutathione remained constant in the organisms from both control and | 164 | glutathione remained constant in the organisms from both control and | ||
| 155 | exposed sites. Although targeted metabolomics has been shown as an | 165 | exposed sites. Although targeted metabolomics has been shown as an | ||
| 156 | early technique of pollutant effect detection, the two-phase pattern | 166 | early technique of pollutant effect detection, the two-phase pattern | ||
| 157 | could highlight a more complicated metabolite response to pollutants | 167 | could highlight a more complicated metabolite response to pollutants | ||
| 158 | than classical biomarkers. (C) 2015 Elsevier B.V. All rights | 168 | than classical biomarkers. (C) 2015 Elsevier B.V. All rights | ||
| 159 | reserved." | 169 | reserved." | ||
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